B5507

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B5507

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Bleomycin sulfate from Streptomyces verticillus

crystalline, 1.5-2.0 units/mg solid

Synonym:Blenoxane, Bleo, Blexane
CAS Number:9041-93-4
EC Number:232-925-2
MDL number:MFCD00070310
PubChem Substance ID:24891839

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Description

ApplicationRecommended for use as a selection agent at 10-100 μg/ml.
Other NotesA mixture of bleomycin sulfate salts
Biochem/physiol ActionsAn antineoplastic antibiotic isolated from Streptomyces verticillus. Binds to DNA, inhibits DNA synthesis and causes DNA scissions at specific base sequences. Needs to bind oxygen and a metal ion such as copper or iron to cleave DNA. Highly selective cleavage of RNA. Inducer and regulator of apoptosis in a variety of cells. Inhibits tumor angiogenesis.

Properties

formcrystalline
color white
solubilityH2O: 20 mg/mL
cation tracesCu: <0.1%
storage temp.2-8°C

Safety

Personal Protective EquipmentEyeshields, Gloves, type P2 (EN 143) respirator cartridges
Hazard CodesT
Risk Statements46-40
Safety Statements53-36/37-45
WGK Germany3
RTECSEC5991990

References

referenceHuttenhofer, A., et al., Cleavage of tRNA by Fe(II)-bleomycin. J. Biol. Chem. 267, 24471-24475, (1992)
 Holmes, C.E, et al., Fe bleomycin as a probe of RNA conformation. Nucleic Acids Res. 24, 3399-3406, (1996)
 Maggliozzo, R.S, et al., Transfer RNA is cleaved by activated bleomycin. Mol. Pharmacol. 35, 428-432, (1989)
 Morgan, M.A. and Hecht, S.M., Iron (II) bleomycin-mediated degradation of a DNA-RNA heteroduplex. Biochemistry 33, 10286-10293, (1994)
 Hamilton, R.F., et al., Bleomycin induces apoptosis in human alveolar macrophages. Am. J. Physiol. 26, L318-L325, (1995)
 Mir, L.M., et al., Bleomycin: revival of an old drug. Gen. Pharmacol. 27, 745-748, (1996)
 Sam, J.W., et al., Sequence-specific changes in the metal site of ferric bleomycin induced by the binding of DNA. J. Biol. Chem. 273, 16090-16097, (1998)
 Holmes, C.E, et al., On the chemistry of RNA degradation by Fe bleomycin. Bioorg. Med. Chem. 5, 1235-1248, (1997)
 Vernole, P, et al., Induction of apoptosis by bleomycin in resting and cycling human lymphocytes. Mutagenesis 13, 209-215, (1998)
 Christensen, J.G., et al., Regulation of apoptosis in mouse hepatocytes and alteration of apoptosis by nongenotoxic carcinogens. Cell. Growth Differ. 9, 815-825, (1998)
 Araki, T, et al., Changes in c-Jun but not Bcl-2 family proteins in p53-dependent apoptosis of mouse cerebellar granule neurons induced by DNA damaging agent bleomycin. Brain Res. 794, 239-247, (1998)
 Kross, J, et al., Specificity of deoxyribonucleic acid cleavage by bleomycin, phleomycin and tallysomycin. Biochemistry 21, 4310-4318, (1982)
 Kross, J, et al., Structural basis for the deoxyribonucleic acid affinity of bleomycins. Biochemistry 21, 3711-3721, (1982)
 Carter, B.J, et al., Site-specific cleavage of RNA by Fe(II) bleomycin. Proc. Natl. Acad. Sci. U. S. A. 87, 9373-9377, (1990)
 Schirner, M., et al., Antiangiogenic chemotherapeutic agents: characterization in comparison to their tumor growth inhibition in human renal cell carcinoma models. Clin. Cancer Res. 4, 1331-1336, (1998)
MerckMerck 13,1311